The Effect of Acute and Chronic Infection-Induced by AvrA Protein of Salmonella typhimurium on Radical Oxygen Species, Phosphatase and Tensin Homolog, and Cellular Homolog Expression During the Development of Colon Cancer

AIM. The aim of the study was to analyze Avra's effector in inducing cancer stem cells into colon through increased radical oxygen species (ROS), PTEN expression and c-myC as markers tumorigenesis mice model colorectal infected with S. typhimurium.
 METHODS. used balb c induced once a week by 10 mg / mL day AOM for 1-week 12-week treatment period. Isolation typhimurium specific protein had been carried out before being intraperitoneal manner amount 40 50 mL. Propagation ATCC bacteria MacConkey media isolation were administered. sample divided 4 groups, positive control group (group that only exposed azoxymethane (AOM), both AvrA (AOM + AvrA), typhimurium). Blood flow cytometry soft tissue sampling IHC data analysis then conducted.
 RESULTS. results showed there an increase ROS, c-Myc. Increased ROS found period it known such due (45.78 ± 2.93) induction compared AOM, (p <0.05). C-myc at 12th negative control.
 CONCLUSION. Inflammation is triggering factor cancer, which c-Myc increases acute chronic phases.